Oct 21, 2022 · Oct. 21st, 2022. The septa and vial caps are essential parts of HPLC vials. The vial cap serves as a barrier, keeping outside contaminants out of the vial and preserving the integrity of the sample. The septa, a seal between the injection needle and the vial composed of silicone, PTFE, or rubber, ensure accurate and exact sample injection.