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Apr 04, 2018 · High performance liquid chromatography (HPLC) and ultra-high-performance liquid chromatography (UHPLC) are two types of commonly used separation methods using liquid chromatography. HPLC has been around since the 1960s, but UHPLC only first appeared in 2004 when Waters created their own trademarked ultra-performance chromatography (UPLC) systems.
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HPLC uses a liquid mobile phase to separate the sample components. The components are dissolved in a solvent and then forced through the column at high pressure. The components then interact with the stationary phase and exit at different times in the same way as in gas chromatography.
High performance liquid chromatography is a powerful tool in analysis. This page looks at how it is carried out and shows how it uses the same principles as in thin layer chromatography and column chromatography. High performance liquid chromatography is basically a highly improved form of column
The Shim-pack XR-ODS column provides both high-speed and high-resolution analysis without requiring an HPLC system exclusively designed for operation under especially high pressures. The Shim-pack XR-ODS column has a 2.2 µm particle size packing which will greatly shorten the analysis time by up to 1/5th that of a general-purpose column (5 µm
This typically ensures the proper interaction of analytes with the non-polar, hydrophobic particle surface. A C18–bonded silica [sometimes called ODS] is the most popular type of reversed-phase HPLC packing. Table C presents a summary of the phase characteristics for the two principal HPLC separation modes based upon polarity.
High-Performance Liquid Chromatography (HPLC) is a high-resolution technique for separation and quantitation of range of small molecules, biomolecules, and peptides. Ultra-high performance (UHPLC) systems are capable of increased pressure and are designed for increased resolution and performance. Platform availability ranges from all-in-one to
HPLC stands for High Performance Liquid Chromatography. Its earlier name was High Pressure Liquid Chromatography because it involved use of liquid mobile phase requiring higher pressures than gases used in Gas Chromatography.
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factor, and tailing factor of amitriptyline); and shape selec-tivity (bonding density). The term shape selectivity is com-monly used to denote a chromatographic quality exhibited by certain stationary phases for which enhanced separations of geometric isomers may result based on their molecular
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With over 40 years as a leader in LC column technology, you can rely on the quality of Thermo Scientific™ HPLC and UHPLC columns. Our extensive family of products offers a variety of particle sizes and column designs to meet all separation needs, including improved resolution, enhanced sensitivity, faster analysis and consistent performance.
Waters HPLC ColumXterra RP 18 (250mm x 4.6mm x5um) 186000496XTerra HPLC Columns. XTerra Phenyl Column, 125Å, 3.5 µm, 2.1 mm X 100 mm, 1/pk - 186001180: XTerra Phenyl columns are hybrid-based, reversed-phase columns that incorporate phenyl ligand functionality.
