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May 07, 2013 · What is HPLC? High Performance Liquid Chromatograph. The basic question that needs a simple answer is “What is HPLC?” In simple terms HPLC is an analytical technique used for separation of components of organic mixture of compounds when such compounds are nonvolatile, thermally unstable and have relatively high molecular weights.
This typically ensures the proper interaction of analytes with the non-polar, hydrophobic particle surface. A C18–bonded silica [sometimes called ODS] is the most popular type of reversed-phase HPLC packing. Table C presents a summary of the phase characteristics for the two principal HPLC separation modes based upon polarity.
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High performance liquid chromatography is a powerful tool in analysis. This page looks at how it is carried out and shows how it uses the same principles as in thin layer chromatography and column chromatography. High performance liquid chromatography is basically a highly improved form of column
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Manufacturer of laboratory instruments and workstations for use with high performance liquid chromatography (HPLC) systems. Products provide heating from 0 degrees C to 150 degrees C, stirring up to 1000 RPM and data acquisition, recording and analysis.
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We are an independent specialist dealer; therefore, we can provide a great selection of HPLC columns. Our product range covers more than 60,000 various columns of more than 20 different manufactures. HPLC columns differ from different separation principles like normal phase, reverse phase and size exclusion chromatography.
With over 40 years as a leader in LC column technology, you can rely on the quality of Thermo Scientific™ HPLC and UHPLC columns. Our extensive family of products offers a variety of particle sizes and column designs to meet all separation needs, including improved resolution, enhanced sensitivity, faster analysis and consistent performance.
Still now, Waters Corporation is the HPLC pioneer, but there are several other companies that manufacture and sell HPLC systems. Technically speaking, the word LC represents all the Liquid Chromatography, including low pressure LC, however most LC systems used these days are HPLC thus often the word LC is used as comparable as HPLC.
Shodex carries about 1000 different LC columns. In other words, we need to choose a best fit column among these 1000 LC columns. 2. HPLC Separation Ideally, obtained LC separation result should provide a symmetrical peak shape (Figure 2a).
