An alternative is to use buffered mobile phase with pH from 5.0 to 7.0. Ammonium acetate buffer pH 5.8, which is compatible with LCMS, was found to be able iii
Jul 01, 2011 · The ultraviolet detection wavelength was set at 276 nm. The calibration curve was linear over a concentration range of 0.05–5.0 μg/mL. The intra-day and inter-day accuracy for three quality controls (QC) samples was 93.48–107.74% and 92.61–96.58%, respectively; the precision was less than 9%.
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High-Performance Liquid Chromatography - Other HPLC Types Ultra High Performance Liquid Chromatography (uHPLC): Where standard HPLC typically uses column particles with sizes from 3 to 5µm and pressures of around 400 bar, uHPLC use specially designed columns with particles down to 1.7µm in size, at pressures in excess of 1000 bar.
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This SOP is applicable for the procedure to be followed in HPLC analysis and documentation and to ensure that Good Laboratory Practices are followed in the HPLC analysis. 3.0 RESPONSIBILITY Officer/ Executive - Quality Control 4.0 ACCOUNTABILITY Manager – Quality Control. 5.0 PROCEDURE
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(10%/100) X 15.5 = 0.1 X 15.5 = 1.55 The required HLB of our oil phase is the sum of these numbers or 7.85 Let's make the Oil Phase more complex. Let's suppose that your oil phase contains: Soybean (Glycine Soja) Oil 11% Caprylic/Capric Triglyceride 5% Grape (Vitis Vinifera) Seed Oil 1% Jojoba (Buxus Chinensis) Oil 1%
watch-glass. The reaction mixture changed through a purple to a brown color and the reaction product was found to be active (Test 19). Since phosphoric anhydride is a dehydrating agent, it was desirable to determine whether the water formed by the dehydration of the cholesterol had any effect.
This procedure is applicable for of all high performance liquid chromatography installed in quality control laboratory. 3.0 RESPONSIBILITY 3.1 Doing: Technical Assistant 3.2 Checking: Executive/Manager 4.0 ACCOUNTABILITY Head of the Department 5.0 PROCEDURE 5.1 For system cleaning 5.1.1 After completion of analysis, clean the HPLC as follows.
4.10.4 Some common supports are glass or ceramic rods, glass saddles, glass hooks, fluorocarbon plastic strings, and various insulated or coated metallic supports. 4.11 Duration of test. 4.11.1 Although duration of any test will be deter- mined by the nature and purpose of the test» an
A non-ionic superhydrophilic base was chemically bonded to the PP vial surfaces. Fig. 2 Overview of Low-Adsorption Glass Vials Glass Vial (LabTotal Vial) Due to the roughness of typical glass vial surfaces, there is a large surface area in contact with samples. Therefore, the surface area was minimized by optimizing the molding
