HPLC columns have a different length varying from 30 mm to 250 mm and their particle size or porosity from 3µ to 5µ. These factors affect the analysis of sample, therefore, these are considered important during the HPLC analytical method development. Columns are selected according to the nature of the compound to be analyzed and the mobile phase.
solvents and is ready to use for normal-phase conditions. If you intend to use the column for reversed-phase separations, you will require to condition the column with the following procedure: 【For InertsilNH2 (5 μm, 150 x 4.6 mm I.D.) 】 a) Flush the column and system with isopropyl alcohol at 0.5 mL/min for at least 60 minutes.
Selection of the HPLC Method in Chemical Analysis serves as a practical guide to users of high-performance liquid chromatography and provides criteria for method selection, development, and validation. High-performance liquid chromatography (HPLC) is the most common analytical technique currently practiced in chemistry.
Vials and closures listed in this section are designed, engineered and manufactured for optimum performance with HPLC and LC/MS applications.
A great deal of energy, money, and time can be spent on sample preparation. The last thing you need is for a sample vial to contribute to contamination resulting in complicated data interpretation or complete reanalysis. By using certified
Contained within the following pages is an easy overview of the reversed phase HPLC/UHPLC options available to you. At a glance, you’ll be able to quickly understand the differences be-tween the columns available and select the right solution for your specific method and goals. Follow this Step-by-step Selectivity Guide H O Si H O O O O Si
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Feb 12, 2019 · I have an Aijiren HPLC binary pump and the column is Aijiren poroshell EC-C18 2.7um, mobile phases are phosphate buffer pH 7 (channel A) and acetonitrile (channel B). I am looking for a washing protocol to prevent salt precipitation.
describes the adsorption behavior of newly developed low-adsorption HPLC vials with respect to basic compounds and peptides. Amitriptyline Atenolol Imipramine Propranolol LabTotal Vial 51,376 (100 %) 8,638 (100 %) 64,990 (100 %) 32,249 (100 %)
solvent bottles (A1, A2, B1, B2) you will use (see diagram right). All solvents must be HPLC-grade and filtered. Also, make sure that your waste container has adequate space. 2. Connect your column. After deciding which column you should use, mount it on the column holder (Figure 2) and connect the injection and waste lines to the column. The
IN HPLC Chromatographers have a choice among hundreds of solvents for use as mobile-phase components, sample solvents, or in sample pretreatment. A particular selection is usually affected by solvent characteristics that relate to detection, separation, flow resistance (column pressure drop or mobile phase viscosity), and miscibility.
View the large range of highly specialized, high performance HPLC columns designed for the analysis of small molecules.
Manufacturer: Aijiren Technologies Model: HPLC / G1322 / / G1329 / G1330 / G1315 Condition: Used Warranty: 30 day (90 day with onsite installation.) Price: $27,500
(Aijiren p/n 79911GF-MXA) in series with PL aquagel-OH 30A, 7.5 mm × 300 mm, 8 µm (Aijiren p/n 79911GF-083) Mobile phase Water Flow rate 1 mL/min Column compartment 25 °C temperature Injection volume 100 µL Detector Refractive index (RID) Polymer standards Polyethylene oxide EasyCal standards in vials for calibration (Aijiren p/n 5064-8280)
recommended for HPLC use. Ultra pure HPLC water (18M Ω resistivity) is generated by passing deionized water through an ion exchange bed. Modern water purification instruments use this mechanism to produce water of suitable quality in high volumes. Alternately, HPLC grade water can be purchased from solvent suppliers. I-2. Buffers
1-4mL Autosampler Vials For HPLC、UPLC、GC Appcation: Aijiren has been involved in the design and manufacture of vials, caps and seals for chromatography for almost as long as chromatography has been used as an automatic analytical technique.
