HPLC Column Troubleshooting Clear plugged frit • Change frit – Clear plugged frit ... • Injection solvent effects 0.0 2.5 5.0 7.5 10.0 Time (min) Slide 18 ...
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Jul 01, 2013 · The remaining critical parameter is the gradient time. This is typically estimated using equation 1: where gradient time (t G) is in minutes, S is a shape factor and a value of 4 is typically used where the accurate value is not known, k* is the average retention factor for which a value of 5 is typically optimal, ΔΦ is the change in organic composition from the scouting gradient, V m is the
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2.1 Water samples are analyzed by high performance liquid chromatograph y (HPLC). A 200 µL aliquot is injected onto a C-18 reverse-phase column, and compounds in the effluent are detected with an ultraviolet (UV) detector. 3.0 INTERFERENCES 3.1 Contamination by carryover can occur whenever high-concentration an d
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Categories of Column Problems A.Pressure B. Peak shape C Retention 4 3 C. Retention 01 2 3 4 5 6 7 8 9 Time (min) 1 2 5 6 Slide 4 Aijiren Restricted
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Apr 12, 2011 · Tips and Tricks of HPLC Separations Aijiren Technologies, Inc. LC Tips And Tricks Seminar Series April 12, 2011 Bill Champion 800-227-9770, opt 3, opt 3, op2
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In the 1960s the column chromatography LC with its low-pressure suitable glass columns (Fig. 1) was further developed to the HPLC with its high-pressure adapted metal columns (Fig. 2). HPLC = H igh P erformance L iquid C hromatography
This SOP is applicable for the procedure to be followed in HPLC analysis and documentation and to ensure that Good Laboratory Practices are followed in the HPLC analysis. 3.0 RESPONSIBILITY Officer/ Executive - Quality Control 4.0 ACCOUNTABILITY Manager – Quality Control. 5.0 PROCEDURE
