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Place insert into autosampler of semi-prepitive HPLC system (Hewlett Packard Series) equipped with an XTerra Prep MS C18 5 μm 7.8x150 mm column and a 100 μL injection loop. Perform multiple 50 μL injections of the sample using a gradient program of 5-95% acetonitrile in water with 0.1% formic acid over 30 minutes while monitoring at 274 nm.
