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LC vials – Chromatography Forum Another point of view for HPLC vial: how tightly should the cap be screwed? We have one method in which methanol is used as diluent and analyte is a volatile solvent. The result is OOS and the root cause may be that the analyte was evaporated from HPLC vial. Our chemist was taught to screw the cap very gently until it cannot be screwed further. China 2ml Screw Thread Clear Glass Vial with Cap and Septa Glass Vial, Vial, Glass Bottle manufacturer / suppl...

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