The Role of Vial Surface Treatment in Minimizing Analyte Adsorption and Sample Loss

Dec 1, 2007 · As the relative loss by adsorption of peptides in low-concentration solutions is more extensive than for more concentrated samples, due to the limiting binding capacity of the wetted solid surface

Only steps 1 and 4 (sample collection and preliminary sample processing) will be briefly explained here. See References 1-4 for an explanation of steps 2, 3, and 5. The bulk of this book will be devoted mainly to Steps 6-9 of Table 1.1, which encompasses what is usually meant by sample pre-treatment or sample preparation (“sample prep”).

May 31, 2019 · Non-specific adsorption (NSA) is a persistent problem that negatively affects biosensors, decreasing sensitivity, specificity, and reproducibility. Passive and active removal methods exist to remedy this issue, by coating the surface or generating surface forces to shear away weakly adhered biomolecules, respectively.

This application note demonstrate that the use of an inappropriate vial can cause analyte adsorption or degradation, potentially leading to out of specification (OOS) results also describes how the type of glass vial selected can impact sample stability, demonstrated for lactone-containing analytes.

Oct 7, 2020 · At low concentrations, analyte adsorption to pipette tips, sample vials and equipment can have significant deleterious effects on signal response. Meanwhile at high concentrations, linearity breaks down due to competitive ionization, signal suppression, and the formation of peptide or protein multimers.

Sep 5, 2014 · Due to the great diversity in physicochemical properties of biomolecules, there is no general approach available to minimize adsorption phenomena. Therefore, we aim to present different strategies which can be generically applied to reduce nonspecific binding of peptides and proteins.

As reported previously, some types of glass vials can lead to in-vial chemical changes of the API. 1–5 Wallace notes that even small quantities of particulate matter on the surface of the vials can have an effect on data quality, including but not limited to irreproducible results or sample loss due to adsorption. 2 Arvary and Mangion

Sep 5, 2014 · To prevent contamination of the next sample via analyte adsorbed at the outer surface of the needle, the exterior of the needle is washed in a vial septum between injections [71]. Consequently, typically low carryover levels are observed, while having a great flexibility in injection volumes.

Step 1. Choose an appropriate container for the analytes of interest Analyte loss from NSB may be due to ionic interactions, hydrophobic interactions, and/or any other interactions. Mitigating NSB thus requires weakening all interactions between the analyte and the surface. Analytes with basic groups → Avoid glass containers

Leave no analyte behind with Waters TruView LCMS certified glass low adsorption autosampler vials - certified/tested for analyte recovery on a UPLC-MS/MS. Learn more.

Aug 2, 2021 · The adsorptive loss of acidic analytes in liquid chromatography was investigated using metal frits. Repetitive injections of acidic small molecules or an oligonucleotide were made on individual 2.1 or 4.6 mm i.d. column frits. Losses were observed for adenosine 5′- (α,β-methylene) diphosphate, 2-pyridinol 1-oxide and the 25-mer

the pharmaceutical ezetimibe.4 Other examinations into the effects of glass vials on sample stability were reported and mitigation strategies proposed by Huang et al.5 These studies demonstrate that the use of an inappropriate vial can cause analyte adsorption or degradation, potentially leading to out of specification (OOS) results.

In contrast, larger and more complex molecules, such as proteins and peptides, may form multiple heterogeneous affinity interactions with the surface to promote adsorption. Changing the composition of sample solutions may weaken one or two affinity mechanisms but may not affect other attractions.

Mar 31, 2015 · As the analyte concentrations are typically low (nM), significant loss of analyte may occur because of nonspecific binding to surfaces other than the intended active one, which may lead to false negative results. Of special concern is the surface of the sample vials as the contact time for the analyte is generally by far the longest there.

Purpose: To evaluate the adsorption effect of a typical basic drug on the surface of glass autosampler vials. Method: Nortriptyline was chosen as a model compound to demonstrate the adsorption effect. The adsorption of this compound in autosampler vials was monitored using LC/MS/MS analysis.